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Old 06/21/2016, 08:56 AM   #51
Shawn O
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Not having another Anemone to do a transplant from, how would I take a donor sample and transport it home without losing the Zooxanthellae? Just in a ziplock full of tank water?


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Old 06/21/2016, 09:19 AM   #52
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I think keeping it from drying out ans use it as soon as you get home would be the thing to do. Make sure you thaw the food in tank water and the temp of the food is at tank temperature to keep the zooxanthellae from getting temperature and salinity shock.


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Current Tank Info: Reboot 320 anemones reef. Angels: Yellow Chest Regal(2), Flame (2). Copperband But. Tangs: Yellow, Purple. Wrasse: about 20 wrasses various species. Anemones: Giantea X4 (Breen, Blue, Purple and Multicolors), Haddoni X1 Red, Magnifica X1 Purpletip
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Old 06/21/2016, 10:01 AM   #53
Shawn O
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OK, great thanks. If my new BTA doesn't color up after a month or two I'll look into this further. Thanks Minh.


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2x Occellaris, 1 Pajama, 1 Neon Dottyback.

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Old 02/03/2017, 07:12 AM   #54
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You could also try phycopure zooxanthelle. I heard its pretty good.


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Old 03/08/2017, 09:59 PM   #55
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What is this "phycopure" of which you speak? I hope it isn't anything like "phocomelia".


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Old 06/08/2017, 12:31 PM   #56
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What is this "phycopure" of which you speak? I hope it isn't anything like "phocomelia".
phycopure zooxanthelle is a bottled live zooxanthelle culture. Google it and you will find many store now sell it


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Old 06/11/2017, 03:37 PM   #57
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I doubt that any zooxanthellae will remain a live in a bottle for very long, if they are alive to begin with.


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Current Tank Info: Reboot 320 anemones reef. Angels: Yellow Chest Regal(2), Flame (2). Copperband But. Tangs: Yellow, Purple. Wrasse: about 20 wrasses various species. Anemones: Giantea X4 (Breen, Blue, Purple and Multicolors), Haddoni X1 Red, Magnifica X1 Purpletip
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Old 07/05/2017, 06:34 PM   #58
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Thank you for sharing this information. This is very interesting. Following!


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Old 02/26/2018, 11:13 AM   #59
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Should I be concerned about this BTA that I have? It was white when I bought it from the store..... Since then it has grown...seems really happy where it is. extends out more, is "bubbly" which I take to be a good sign.... Everything else in my tank seems to be doing great. Water parameters are great. Calcium levels are fine. lighting is great. my other BTA is still colorful and happy.... just worried that i might need to be concerned with this little guys color....


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Old 04/06/2018, 09:36 AM   #60
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I am not sure the actual color of this anemone, however there seem to be a little brown that I see. He will be fine. He is not 100% bleached and should recover given good condition. Update if you can.


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Current Tank Info: Reboot 320 anemones reef. Angels: Yellow Chest Regal(2), Flame (2). Copperband But. Tangs: Yellow, Purple. Wrasse: about 20 wrasses various species. Anemones: Giantea X4 (Breen, Blue, Purple and Multicolors), Haddoni X1 Red, Magnifica X1 Purpletip
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Old 06/23/2019, 02:01 PM   #61
ThRoewer
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Originally Posted by OrionN View Post
I doubt that any zooxanthellae will remain a live in a bottle for very long, if they are alive to begin with.
They can:
http://burr.bio.buffalo.edu/index.php/cultures/


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Old 06/24/2019, 04:53 AM   #62
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Originally Posted by ThRoewer View Post
I am sure that someone will be able to isolate and growth cultures of Zooxanthellae, like the scientists in the link you provided above. However, I do not think that the bottle of

Algagen PhycoPure™ Zooxanthellae (Assorted species) Item: BEH-84761(as per Live Aquaria)
would provide live or usable strains of zooxanthellae as claimed.
I think isolate growing them in control setting is one thing, but then bottle them up and put them on shelf of stores where they are in there, non aerated, no temp control, no light for days, weeks or months; any living organism in there would be long gone by the time we dump it into our tank. My most likely guess would be we just dump some expensive dirty water into our tank.


While I maybe completely wrong about this but I have attempt to keep living culture of various organism before. Each organism does have very specific condition where it thrive at. For cultures of this sort, having verified viable sample, transport overnight in thermally isolated condition is likely need to keep it viable. Also, there are a numbers of Zooxanthellae strains. Those that living in one coral, even of the same species, may not be the same as those that live in another coral. There also evidence of a numbers of species of Zooxanthellae living in the same host.


This is part of the disclaimer of the Scientist that culture these zooxanthellae. If you want to request a sample, you must agree in written before they transfer a sample of these zooxanthellae to you:



Transfer of BURR Cultures Agreement FormName: ________________________________________________________________Email address: __________________________________________________________Phone number: __________________________________________________________Address to ship samples:FedEX (or other overnight courier) Number: _________________________________List of cultures requested:Culture IDClade/cp-type*Culture ID Clade/cp-type**cp-type- symbiont strain based on fragment size of hypervariable region of chloroplast 23S rDNACost - $90.00 per culture plus a $13.00 administrative fee Summary of intended use of cultures:You acknowledge that many of our cultures are not axenic or well characterized. Furthermore, our cultures are not necessarily representative of the symbionts within the host from which they were isolated. We have verified this for somecultures, but not for all. This point is important when reporting any findings from these cultures. However, if you agree to several things, then I am happy to authorize the transfer


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Current Tank Info: Reboot 320 anemones reef. Angels: Yellow Chest Regal(2), Flame (2). Copperband But. Tangs: Yellow, Purple. Wrasse: about 20 wrasses various species. Anemones: Giantea X4 (Breen, Blue, Purple and Multicolors), Haddoni X1 Red, Magnifica X1 Purpletip
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Old 06/24/2019, 11:22 AM   #63
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Quote:
Originally Posted by OrionN View Post
I am sure that someone will be able to isolate and growth cultures of Zooxanthellae, like the scientists in the link you provided above. However, I do not think that the bottle of

Algagen PhycoPure™ Zooxanthellae (Assorted species) Item: BEH-84761(as per Live Aquaria)
would provide live or usable strains of zooxanthellae as claimed.
I think isolate growing them in control setting is one thing, but then bottle them up and put them on shelf of stores where they are in there, non aerated, no temp control, no light for days, weeks or months; any living organism in there would be long gone by the time we dump it into our tank. My most likely guess would be we just dump some expensive dirty water into our tank.
The first bottle I got from LiveAquaria was dead. But then Algagen sent me a fresh replacement and the algae in that one were alive.
However, they failed to infect the anemone I had gotten them for.
So I would say it is rather unlikely to get a benefit from these whether they are alive or not.


Quote:
Originally Posted by OrionN View Post

While I maybe completely wrong about this but I have attempt to keep living culture of various organism before. Each organism does have very specific condition where it thrive at. For cultures of this sort, having verified viable sample, transport overnight in thermally isolated condition is likely need to keep it viable. Also, there are a numbers of Zooxanthellae strains. Those that living in one coral, even of the same species, may not be the same as those that live in another coral. There also evidence of a numbers of species of Zooxanthellae living in the same host.

...
There has and still is a lot of research done on this in regards to coral bleaching and the results are so far that there are some coral that have a very specific Symbiodinium mix while others are open to a large variety of types and strains.
Given that host anemones are more scattered over the reefs and in the high intensity light range I could see them to be less specific. For example, I'm quite certain that a bleached crispa I once had acquired symbionts from a neighboring Anthelia colony. The re-colonization/colorization started precisely in those tentacles that frequently got in contact with the Anthelia. So it didn't only cross a species barrier but went to a quite distant genus.
Geographic origin may possibly have more to do with it than species.


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Pairs: 4 percula, 3 P. kauderni, 3 D. excisus, 1 ea of P. diacanthus, S. splendidus, C. altivelis O. rosenblatti, D. janssi, S. yasha & a Gramma loreto trio
3 P. diacanthus. 2 C. starcki

Current Tank Info: 200 gal 4 tank system (40x28x24 + 40B + 40B sump tank + 20g refugium) + 30x18x18 mixed reef + 20g East Pacific biotop + 20g FW +...
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Old Yesterday, 03:13 AM   #64
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So I got a new magnifica that isn't bleached and took 2 or 3 tentacles from it and injected them with a syringe into the gastral cavity of my bleached magnifica. The tentacles stayed wet all the time and it took only a couple of seconds from harvest to injection.

I have to go with injecting the transplants this way since the magnifica doesn't have a strong enough feeding reflex to actually grab food and put it into her mouth. Aside from that, the bicinctus steal or fan away any food I put into the anemone.

The injection was a week ago but so far I can't see any success of the procedure.
To make matters worse, the new anemone has started deflating so I will likely have to treat it ... which may lead to it bleaching as well...

I had tried feeding my other bleached anemone, a gigantea from LA, a few tentacles from my healthy giganteas about a months ago, but the transfer also didn't take.


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Pairs: 4 percula, 3 P. kauderni, 3 D. excisus, 1 ea of P. diacanthus, S. splendidus, C. altivelis O. rosenblatti, D. janssi, S. yasha & a Gramma loreto trio
3 P. diacanthus. 2 C. starcki

Current Tank Info: 200 gal 4 tank system (40x28x24 + 40B + 40B sump tank + 20g refugium) + 30x18x18 mixed reef + 20g East Pacific biotop + 20g FW +...
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